vu bio203 Final Term - Quiz No.3
vu bio203 Methods in Molecular Biology Quiz
This quiz belongs to book/course code vu bio203 Methods in Molecular Biology of vu organization. We have 4 quizzes available related to the book/course Methods in Molecular Biology. This quiz has a total of 10 multiple choice questions (MCQs) to prepare and belongs to topic Final Term. NVAEducation wants its users to help them learn in an easy way. For that purpose, you are free to prepare online MCQs and quizzes.
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Question 1: A heat stable enzyme called Taq Polymerase is used in Polymerase Chain Reaction (PCR) which is obtained from a bacterium __________.
Escherichia coli
Salmonella Typhi
Thermus aquaticus
Actinobacillus capsulatus
Question 2: A technique for making millions of copies of a specific region of DNA.
DNA ligase
Restriction enzymes
Gel electrophoresis
Polymerase chain reaction
Question 3: Which of the following statements are true regarding PCR?
Primer extension occurs at 72°C
Denaturation involves heating at 90 to 98°C
Annealing involves the binding of primer between 40 to 60°C
All of the given
Question 4: In __________ PCR there is very low probability of non-specific amplification of DNA.
Nested
Multiplex
RT-PCR
Conventional
Question 5: Inverse PCR supports the amplification and identification of __________.
Flanking sequences
Genomic inserts
Transposable elements
All of the given
Question 6: Primers used for the process of polymerase chain reaction are __________.
Single-stranded RNA oligonucleotide
Single-stranded DNA oligonucleotide
Double-stranded RNA oligonucleotide
Double-stranded DNA oligonucleotide
Question 7: Denaturation is the process of __________.
Heating between 72°C
Heating between 40 to 60°C
Heating between 90 to 98°C
All of the given
Question 8: Why are vent ploymerase and Pfu more efficient than the Taq polymerase?
Because of proofreading activity
Because of more efficient polymerase activity
Both of the given
None of the given
Question 9: Which one of the following is the source of pfu Polymerase?
Thermococcus litoralis
Thermococcus litoralis
Pyrococcus furiosus
T. aquaticus
Question 10: Two pair of enzymes are used in __________ PCR.
Nested
Multiplex
RT-PCR
Conventional
